SaMag-12™ System

Automated Nucleic Acid Extraction System from Sacace Biotechnologies

The SaMag-12™ is a compact bench-top extractor for automated nucleic acid purification. It processes 1–12 samples in parallel, recommended for labs with small-medium sample throughputs.

Features & Advantages

  • Fully automated high yield nucleic acid extraction with magnetic beads and PCR setup (for Sacace kits like HCV, HBV)
  • Reagent identification with on-board barcode scanners
  • Pre-programmed protocols
  • Flexible batch size from 1 up to 12 samples in parallel
  • All required tubes, tips, plastics are inside the provided Extraction Kits
  • Ready to use reagents
  • Very fast extraction protocol (~40 minutes)
  • Very simple operation (easy to install, operate, maintain)
  • Isolation of very pure nucleic acids

Ease of use

3 EASY STEPS to purify nucleic acids from different sample materials

Complete solution containing everything needed for purification: prefilled, film-sealed reagent cartridges, disposable plastics, reaction and elution tubes. At the end of the process, purified nucleic acids are auto collected into 1.5 ml tubes for storage or directly into 0.2 ml PCR tubes thus including PCR setup (for example using Sacace HBV and HCV CE-IVD marked detection kits).

Technology

Magnetic bead extraction

Purification process includes steps of lysis, binding, washing and elution.

Pestle Mixing

When required, the pestle agitate and mix very well the liquid, avoiding clogged tips problem and ensuring good nucleic acid extraction even for highly viscous specimens.

Polygon Reaction Chamber

Ensure highest efficiency of lysis and elution, greatly minimizing the presence of magnetic beads and alcohol residues in the final eluted sample.

Performance

High Stability and Reproducibility

Twenty replicates were performed on SaMag™ nucleic acid purification system. The Real-Time qPCR data shows very high reproducibility.

Linearity

A dilution series (from 8,00 log IU/ml to 1,00 log IU/ml) of an HCV RNA synthetic quantitative standard calibrated against the 4th WHO International HCV RNA Standard was extracted with SaMag™ system and tested using Real Time PCR, showing extremely wide linear range.

Consistent Yield

Genomic DNA extracted from human whole blood samples. Electrophoresis image shows high consistency and reproducibility on extraction results.

Sensitivity

Real Time PCR used to calculate sensitivity of SaMag™ Viral Nucleic Acid extraction kit. Results show excellent sensitivity of 15 IU/ml that can be detected when using Sacace HBV
CE-IVD marked detection kit.

Technical Specifications

ParameterSaMag-12™ 
Extraction MethodMagnetic Beads – binding, washing, elution
Throughput1–12 samples/run 
DisplayLCD 20×4 characters
Heat Block TemperatureRT – 65 °C
Dimensions (W×D×H)48 × 70 × 52 cm 
Weight43 kg
Operating Temperature15–30 °C
Operating Humidity30–80% (non condensing)
Electrical110–240 V, 50/60 Hz
Complete Automation: performing PCR setup

Performing PCR Setup

Using a dedicated sample rack, it is possible to automate not only the extraction process but also the PCR setup phase. The eluted product is automatically collected in 0.2 ml PCR tubes already containing lyophilized PCR reagents and ready to be transferred into Real Time instrument to perform amplification.

Valid when using Sacace HBV and HCV CE-IVD marked detection kits.

SaMag Viral Nucleic Acids Extraction kit

Intended Use

SaMag Viral Nucleic Acid Extraction Kit is used with the SaMag-12 or SaMag-24 instruments for extraction of Viral DNA or RNA from human biological specimens such as serum, plasma, and other cell-free fluids. 400 µl of plasma was spiked with serial-diluted Hepatitis C Virus (in range of 5000-50 IU /µl) extracted and amplified with Real Time PCR, proving the excellent sensitivity and linearity of isolation procedure.

Application

Nucleic acids extracted from SaMag Viral Nucleic Acid Extraction kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis. 400 µl of plasma was spiked with serial-diluted Hepatitis C Virus (in range of 5000-50 IU /µl) extracted and amplified with Real Time PCR, proving the excellent sensitivity and linearity of isolation procedure.
SaMag MTB DNA Extraction Kit

Intended Use

Genomic DNA extracted from human whole blood samples. Electrophoresis image shows high consistency and reproducibility on extraction results.

Application

Nucleic acids extracted from TB DNA Extraction kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.

Using SaMag™ TB DNA Extraction kit to isolate DNA from clinical specimens (sputum, CSF and PAS). 100 µl sample used for extraction and collect 100 µl eluate. Analysis was performed by real-time qPCR (IS611O detection). Even in the cell-free body fluid (CSF) and blood contaminated sample (PAS), the TB DNA can be detected after extraction, proving the excellent sensitivity of isolation procedure.

Extraction kits reagents (to use with SaMag™ system)
Product Cat. Type Composition Description
SaMag™ Viral Nucleic Acid Extraction Kit SM003 CE-IVD 1 kit (48 extr.) incl. plastics Extraction of viral nucleic acids from plasma, serum or cell-free body fluids
SaMag™ Bacterial DNA Extraction Kit SM006 CE-IVD 1 kit (48 extr.) incl. plastics Extraction of genomic DNA from Gram+ and Gram− bacteria
SaMag™ STD DNA Extraction Kit SM007 CE-IVD 1 kit (48 extr.) incl. plastics STD DNA (Chlamydia, Neisseria, HPV…) from swabs, urine, seminal liquid
SaMag™ TB DNA Extraction Kit SM008 CE-IVD 1 kit (48 extr.) incl. plastics Mycobacterium tuberculosis DNA from clinical specimen or culture
SaMag™ Tissue DNA Extraction Kit SM004 CE-IVD 1 kit (48 extr.) incl. plastics Genomic DNA from a variety of tissues
SaMag™ Blood DNA Extraction Kit SM001 CE-IVD 1 kit (48 extr.) incl. plastics Genomic DNA from whole blood, PBMC or buffy coat
SaMag™ FFPE DNA Extraction Kit SM009 CE-IVD 1 kit (48 extr.) incl. plastics Genomic DNA from FFPE samples

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